Alejandro Reyes Pinto

Academic Training

  • Licenciado en Ciencias c/m Biología, U. de Chile, 1982
  • Doctor en Ciencias c/m Biología, U. de Chile, 1990
  • PostDoctorado Bioquímica, University of Missouri – Kansas City, USA, 1990-1993

 

Scientific Interests

Proteins within a cell are the macromolecules that carry out specific biological processes. Through proteins, cells can breakdown food substances to obtain energy, communicate and interact with the environment and replicate their genetic material. Protein engineering is a fascinating mixture of molecular biology, protein structure studies, computing and biochemistry. This interaction makes it possible to incorporate site-specific mutations into the genes of any protein, which in turn makes it possible to study the role of any amino acid or group of amino acids if the mutant protein can be produced in the appropriate expression system.
Our group has been interested in developing a line of advanced research on protein structure and function. As our study system, we have been using integral membrane proteins that behave as facilitative transporters. We are currently interested in the regulation of hexose transporter activity, by both natural and synthetic compounds. For this, we are using a variety of biochemical and biophysical methods, including reconstitution of purified proteins, site-directed mutagenesis, heterologous expression, fluorescence spectroscopy and bioinformatics. Likewise, we are interested in applying modern protein engineering strategies (directed evolution, drug design) to design enzymes of industrial interest.
 

Proyects:

  • FONDEF D07i1117 (2008-2013). Innovar en las estrategias terapéuticas para el tratamiento de la diabetes a través de un curso de acción complementario a la insulina. Desarrollo de moléculas activadoras con especificidad para sitios regulatorios de los transportadores de glucosa
  • FONDEF D11I1131 (2012-2115). Identificación, desarrollo y validación de nuevas moléculas inhibidoras con selectividad para los transportadores de glucosa para su aplicación en el control de la hiperglicemia característica de la diabetes mellitus, obesidad y síndrome metabólico
  • FONDEF CA12I10022 (2013-2015). Desarrollo de un preparado de fitasa activa a bajas temperaturas (10-16°C) para suplementar in vivo dietas de peces con altos niveles de proteína vegetal
  • FONDECYT 1130386 (2013-2017). Structure-functional biology of GLUT1 and GLUT2 human hexose transporters
  • DID-UACh S-2012-46 (2012-2014). Selección de microrganismos nativos productores de fitasa con actividad a +14°C y determinación de las condiciones óptimas de producción de la enzima para el escalamiento
  • DID-UACh S-2013-22 (2013-2015). Entendiendo la farmacología molecular de resveratrol y NDGA en células leucémicas humanas.
  • DID-UACh  S-2013-20 (2013-2015). GLUT12: Transportador de hexosas en la interface SGLT/GLUT

 

Relevant Publications

  • Vera, J.C., Reyes, A.M., Velásquez, F.V., Rivas, C.I., Zhang, R.H., Strobel, P., Slebe, J.C., Núñez-Alarcón, J. y Golde, D.W. (2001). Direct inhibition of the hexose transporter GLUT1 by tyrosine kinase inhibitors. Biochemistry 40, 777-790.
  • Reyes, A.M., Bustamante, F., Rivas, C.I., Ortega, M., Donet, C., Rossi, J.P., Fischbarg, J. y Vera, J.C. (2002). Nicotinamide is not a substrate of the hexose transporter GLUT1. Biochemistry 41, 8075-8081.
  • Reyes, A.M., Ludwig, H.C., Yañez, A., Rodríguez, P.H. y Slebe, J.C. (2003). Native-like intermediate in the unfolding pathway of pig kidney fructose-1,6-bisphosphatase. Biochemistry 42, 6956-6964.
  • Rodríguez, P., Rivas, C.I., Godoy, A., Villanueva, M., Fischbarg, J., Vera, J.C. y Reyes, A.M. (2005). Redefining the Facilitated Transport of Mannose in Human Cells: Absence of a Glucose-Insensitive, High-Affinity Facilitated Mannose Transport System. Biochemistry 44, 313-320.
  • Godoy, A., Ormazabal, V., Moraga-Cid, G., Zúñiga, F.A., Sotomayor, P., Barra, V., Vasquez, O., Montecinos, M., Mardones, L., Guzmán, L., Villagrán, M., Aguayo, L.G., Oñate, S.A., Reyes, A.M., Cárcamo, J.G., Rivas, C.I. y Vera, J.C. (2007). Mechanistic insights and functional determinants of the transport cycle of the ascorbic acid transporter SVCT2. Activation by sodium and absolute dependence on bivalent cations. J. Biol. Chem. 282, 615-624.
  • Pérez, A.A., Ojeda, P.G., Valenzuela, X.E., Ortega, M.S., Sánchez, C., Ojeda, L., Castro, M.A., Cárcamo, J.G., Rauch, C., Concha, I.I., Rivas, C.I., Vera, J.C. y Reyes, A.M. (2009). Endofacial competitive inhibition of the glucose transporter-1 activity by gossypol. Am J Physiol Cell Physiol 297, 86-93, 2009
  • Costa, M., Torres, M., Magariños, H. y Reyes, A.M.  (2010). Producción y purificación parcial de enzimas hidrolíticas de Aspergillus ficuum en fermentación sólida sobre residuos agroindustriales. Rev. Colomb. Biotecnol. 12, 163-175
  • Ormazabal, V., Zuñiga, F.A., Escobar, E., Aylwin, C., Salas-Burgos, A., Godoy, A., Reyes, A.M., Vera, J.C. y Rivas, C.I. (2010). Histidine Residues in the Na+-coupled Ascorbic Acid Transporter-2 (SVCT2) are Central Regulators of SVCT2 Function, Modulating pH Sensitivity, Transporter Kinetics, Na+ Cooperativity, Conformational Stability, and Subcellular Localization. J. Biol. Chem. 285, 36471-36485.
  • Pérez, A., Ojeda, P., Ojeda, L., Salas, M., Rivas, C.I., Vera, J.C. y Reyes, A.M. (2011). Hexose transporter GLUT1 harbors several distinct regulatory binding sites for flavones and tyrphostins. Biochemistry 50, 8834-8845.
  • Mardones, L., Zúñiga, F.A., Villagrán, M., Sotomayor, K., Mendoza, P., Escobar, D., González, M., Ormazábal, V., Maldonado, M., Oñate, G., Angulo, C., Concha, I.I., Reyes, A.M., Cárcamo, J.G., Barra, V., Vera, J.C. y Rivas, C.I. (2012). Essential role of intracellular glutathione in controlling ascorbic acid transporter expression and function in rat hepatocytes and hepatoma cells. Free Radic. Biol. Med. 52, 1874-1887.
  • Ojeda, P., Pérez, A., Ojeda, L., Vargas-Uribe, M., Rivas, C.I., Salas, M., Vera, J.C. y Reyes, A.M. (2012). Non-competitive blocking of human glut1 hexose transporter by methylxanthines reveals an exofacial regulatory binding site. Am. J. Physiol. Cell Physiol. 303, C530 –C539.
  • Marcia Costa, Marcelo Torres y Alejandro Reyes. (2012). Caracterización de enzimas hidrolíticas de Aspergillus ficuum producidas en fermentación sólida sobre torta de canola. Rev. Colomb. Biotecnol. 14, 208-215.
  • Salas M, Obando P, Ojeda L, Ojeda P, Vargas U, Rivas C, Vera JC, Reyes AM (2013). Resolution of the direct interaction and inhibition of the human GLUT1 hexose transporter by resveratrol from its effect on glucose accumulation. Am J Physiol Cell Physiol ajpcell.00387.2012; published ahead of printApril 24, 2013, doi:10.1152/ajpcell.00387.2012