Academic Training

- Scientific Baccalauréat, Pharmacy and Biochemistry, Universidad Católica Santa María de Arequipa, Perú 1992
- Pharmaceutical Chemist, Universidad Católica Santa María de Arequipa, Perú 1992
- Masters degree, Biochemistry, Universidad de Concepción, 1998
- Ph.D., Cell and Molecular Biology, Universidad de Concepción 2004
- Postdoctorate, Universidad Austral de Chile, 2006-2008


Scientific Interests

My postdoctoral research project is focused on the “search for early markers of diabetic nephropathy”. It is known that in diabetes, there is a 300% increase in endogenous glucose production in kidney, compared to only a 30% hepatic increase. The heightened increase in kidney might be responsible for the stimulation and accumulation of glycogen, typically observed in diabetic nephropathies. Rats with diabetes, induced by short-term treatment of streptozotocin, show a decrease in renal and hepatic glycogen content. However, long-term treatment (9 months) reveals that large quantities of glycogen are accumulated, and that in contrast to short-term rats, this glycogen may be hydrolyzed to produce glucose. It is of interest to mention that this increase in glycogen formation also occurs in other significant organs, such as the pancreas. These findings highlight the relevance of a study in which glucose synthesis is induced in a diabetic animal model over the short- medium- and long-term. We are interested in studying a possible integration between gluconeogenesis and glycogen synthesis, as well as the respective signal pathways. The increase in gluconeogenic enzyme expression and its later release into the urine may allow us to use these metabolism proteins as possible early (response) markers in diabetic nephropathy.

Relevant Publications

- Uribe, E., Salas, M., Enriquez, S., Orellana, MS, Carvajal, N. Cloning and functional expresión of a rodent brain cDNA encoding a novel protein with agmatinase activity, but not belonging to the arginase family. Arch Biochem and Biophys (2007)

- Carvajal, N., Orellana, M.S., Salas, M., Enriquez, P., Alarcón, R., Uribe, E., López V. Kinetic studies and site-directed mutagenesis of Escherichia coli agmatinase. A role for Glu274 in binding and correct positioning of the substrate guanidinium group. Arch Biochem Biophys. 430, 185-190. (2004)

- Carvajal, N., Orellana, M.S., Bórquez, J., Uribe, E., Lopez, V., Salas, M. Non-chelating inhibition of the H101N variant of human liver arginase by EDTA. J Inorg Biochem. 98, 1465-1469. (2004)

- Carvajal, N., Uribe, E., Lopez, V., Salas, M. Inactivation of human liver arginase by Woodward's reagent K: evidence for reaction with His141. Protein J. 23, 179-183. (2004)

- Salas, M., López, V., Uribe, E., Carvajal, N. Studies on the interaction of Escherichia coli agmatinase with manganese ions: structural and kinetic studies of the H126N and H151N variants. J Inorg Biochem. 98, 1032-1036 (2004)

- Salas, M., Rodríguez, R., Uribe, E., López, V., Fuentes, M., López, N., Carvajal, N. (2002) Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling. Eur. J. Biochem. 269, 5522-5526

Photo Gallery

Foto 1 Foto 2 Análisis anatomo-patológico de riñon de ratas diabéticas tratadas con STZ

Phone: 221332 / Fax: 221107
Campus Isla Teja / Valdivia

- Enzyme regulation of glucidic metabolism
- Search for diabetic nephropathy markers

- Dr. Juan Carlos Slebe
- Dr. Alejandro Yañez
- Dr. Heide Ludwig

Postgraduate Students:
- Joel L. Asenjo
- Romina Bertinat
- Carlos Spichiger
- Rodrigo Gatica
- Zahady D. Velásquez
- Marco A. Maureira
- Fabián N. Pardo

Undergraduate Students:
- Karen A. Jaramillo
- Juan P. Póntigo
- Luciano A. Rivera
- Guillermo S. Triviño
- Joaquín Vivar

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